Sains Malaysiana 53(5)(2024): 1093-1104

http://doi.org/10.17576/jsm-2024-5305-10

 

Morphological and Molecular Characterization of Spirogyra Species from Water Bodies in Chiang Rai Province, Thailand: Insights into Bioactivity and Antioxidant Potential

(Pencirian Morfologi dan Molekul Spesies Spirogyra daripada Jasad Air di Wilayah Chiang Rai, Thailand: Pandangan tentang Bioaktiviti dan Potensi Antioksidan)

 

KITTIYA PHINYO1,2, KUHLAB SAEKU3, WIPAWAN NUNTO 3, JEERAPORN PEKKOH1 &

TIPPAWAN PRASERTSIN3,*

 

1Biology Department, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand

2Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand

3Biological Science Program, Faculty of Science and Technology, Chiang Rai Rajabhat University, Chiang Rai 57100, Thailand

 

Diserahkan: 2 April 2023/Diterima: 18 Mac 2024

 

Abstract

This study aimed to identify Spirogyra species collected from lentic (sampling site code CR01) and lotic (sampling site code CR02) water areas in Chiang Rai Province, Thailand. The diagnosis was proceeded by studying the morphological characteristics combined with molecular techniques with the chloroplast rbcL gene (ribulose bisphosphate carboxylase). Additionally, the bioactive properties of Spirogyra extracts were evaluated through antimicrobial and antioxidant assays. The extraction was performed with water and ethanol extraction. The results indicated that the morphological characteristics of Spirogyra CR01 resembled Spirogyra neglecta, but the data of rbcL gene for this species were not available in the database. Therefore, the Spirogyra CR01 sequence was added to the GenBank database, representing the report of rbcL gene of Spirogyra neglecta sequencing data. Spirogyra CR02 was robustly identified as S. fluviatilisthrough molecular analysis. The extracts from both Spirogyra samples showed that the ethanolic extract has more activity than the water extract. The antimicrobial ability of the ethanolic extract from Spirogyra CR02 was observed to inhibit the growth of Bacillus cereus and Staphylococcus aureus, while the ethanolic extract from Spirogyra CR01 only inhibited B. cereus. Moreover, the ethanolic extract of Spirogyra CR01 had statistically significant (p<0.05) antioxidant activity, with the highest value recorded at 114.92±2.38 mg GAE/g extract. Overall, this study provides new sequencing information on the S. neglecta and their potential for use Spirogyra in functional food, functional ingredients and cosmeceutical products.

 

Keywords: Antioxidant; bioactive compound; lentic water; lotic water; rbcL gene

 

Abstrak

Penyelidikan ini bertujuan untuk mengenal pasti spesies Spirogyra yang dikumpul dari kawasan perairan lentik (kod tapak persampelan CR01) dan lotik (kod tapak persampelan CR02) di Wilayah Chiang Rai, Thailand. Diagnosis diteruskan dengan mengkaji ciri morfologi yang digabungkan dengan teknik molekul dengan gen rbcL kloroplas (ribulose biphosphate carboxylase). Selain itu, sifat bioaktif ekstrak Spirogyra telah dinilai melalui ujian antimikrob dan antioksidan. Pengekstrakan dilakukan dengan pengekstrakan air dan etanol. Keputusan menunjukkan bahawa ciri morfologi Spirogyra CR01 menyerupai Spirogyra neglecta, tetapi data gen rbcL untuk spesies ini tidak tersedia dalam pangkalan data. Oleh itu, jujukan Spirogyra CR01 telah ditambahkan pada pangkalan data GenBank, mewakili laporan gen rbcL data penjujukan Spirogyra neglecta. Spirogyra CR02 telah dikenal pasti sebagai S. fluviatilis melalui analisis molekul. Ekstrak daripada kedua-dua sampel Spirogyra menunjukkan bahawa ekstrak etanol mempunyai lebih aktiviti daripada ekstrak air. Keupayaan antimikrob ekstrak etanol daripada Spirogyra CR02 diperhatikan untuk merencat pertumbuhan Bacillus cereus dan Staphylococcus aureus, manakala ekstrak etanol daripada Spirogyra CR01 hanya merencat B. cereus. Selain itu, ekstrak etanol Spirogyra CR01 mempunyai aktiviti antioksidan yang signifikan secara statistik (p<0.05), dengan nilai tertinggi direkodkan pada 114.92±2.38 mg ekstrak GAE/g. Secara keseluruhannya, kajian ini menyediakan maklumat penjujukan baharu tentang S. neglecta dan potensi mereka untuk menggunakan Spirogyra dalam makanan berfungsi, ramuan berfungsi dan produk kosmeseutikal.

 

Kata kunci: Air lentik; air lotik; antioksidan; gen rbcL; sebatian bioaktif

 

RUJUKAN

Ahmed, N., Karobari, M.I., Yousaf, A., Mohamed, R.N., Arshad, S., Basheer, S.N., Peeran, S.W., Noorani, T.Y., Assiry, A.A., Alharbi, A.S. & Yean, C.Y. 2022. The antimicrobial efficacy against selective oral microbes, antioxidant activity and preliminary phytochemical screening of Zingiber officinale. Infection and Drug Resistance 15: 2773-2785.

Al-Garadi, M.A., Qaid, M.M., Alqhtani, A.H., Alhajj, M.S., Al-abdullatif, A.A. & Al-Mufarrej, S.I. 2022. In vitro antimicrobial efficacy assessment of ethanolic and aqueous extracts of Cinnamon (Cinnamomum verum) bark against selected microbes. Brazilian Journal of Poultry Science 25(1): 001-016.

Alshididi, S.S. & Jawad, L.M. 2015. Antagonistic activity of Spirogyra micropunctata against some multidrug resistant human pathogenic bacteria. Iraqi Journal of Science 56(3): 2494-2498.

Bailey, R. 2020. Gram positive vs. Gram negative bacteria. ThoughtCo. https://www.thoughtco.com/gram-positive-gram-negative-bacteria-4174239 Accessed 17 February 2021.

Borchardt, M.A. 1994. Effects of flowing water on nitrogen and phosphorus limited photosynthesis and optimum N: P ratios by Spirogyra fluviatilis (Charophyceae). Journal of Phycology 30(3): 418-430.

Brand-Williams, W., Cuvelier, M.E. & Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology 28(1): 25-30.

Chairerk, N., Pongyeela, P. & Chungsiriporn, J. 2021. The studies also suggested that ethanol is the most suitable nontoxic solvent for extracting the compounds. Applied Science and Engineering 14(1): 52-59.

Chen, C., Barfuss, M.H.J., Pröschold, T. & Schagerl, M. 2012. Hidden genetic diversity in the green alga Spirogyra (Zygnematophyceae, Streptophyta). BMC Evolutionary Biology 12: 77.

Chowdhury, M.M.H., Kubra, K., Hossain, M.B., Mustafa, M.G., Jainab, T., Karim, M.R. & Mehedy, M.E. 2015. Screening of antibacterial and antifungal activity of freshwater and marine algae as a prominent natural antibiotic available in Bangladesh. International Journal of Pharmacology 11(7): 828-833.

Chun-Hoong Ho, Noryati Ismail, Shaida-Fariza Sulaiman & Rosma Ahmad. 2001. In vitro antibacterial and antioxidant activities of Orthosiphon stamineus Benth. Food Chemistry 122(4): 1168-1172.

Dai, J. & Mumper, R.J. 2010. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 15: 7313-7352.

Delfanian, M., Kenari, R.E. & Sahari, M.A. 2015. Influence of extraction techniques on antioxidant properties and bioactive compounds of loquat fruit (Eriobotrya japonica Lindl.) skin and pulp extracts. Food Science & Nutrition 3(3): 179-187.

Do, Q.D., Angkawijaya, A.E., Tran-Nguyen, P.L., Huynh, L.H., Soetaredjo, F.E., Ismadji, S. & Ju, Y.H. 2014. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis 22(3): 296-302.

Drummond, C.S., Hall, J., Karol, K.G., Delwiche, C.F. & McCourt, R.M. 2005. Phylogeny of Spirogyra and Sirogonium (Zygnematophyceae) based on rbcL sequence data. Journal of Phycology 41(5): 1055-1064.

Duangjai, A., Limpeanchob, N., Trisat, K. & Amornlerdpison, D. 2016. Spirogyra neglecta inhibits the absorption and synthesis of cholesterol in vitro. Integrative Medicine Research 5: 301-308.

Dwaish, A.S., Yousif, D.Y.M. & Lefta, S.N. 2016. Use of Spirogyra sp. extract against multidrug resistant bacterial pathogens. International Journal of Advanced Research 4(7): 575-579.

Guiry, M.D. & Guiry, G.M. 2020. AlgaeBase. World-wide electronic publication. National University of Ireland, Galway. https://www.algaebase.org Accessed 11 November 2020.

Hainz, R., Wöber, C. & Schagerl, M. 2009. The relationship between Spirogyra (Zygnematophyceae, Streptophyta) filament type groups and environmental conditions in Central Europe. Aquatic Botany 91(3): 173-180.

Hamdam, N., Samad, A.A., Hidyat, T. & Salleh, F.M. 2013. Phylogenetic analysis of eight Malaysian pineapple cultivars using a chloroplast marker (rcbL gene). Jurnal Teknologi64: 29-33.

Jaya Prakash Goud, M., Seshikala, D. & Mharya, A.S.C. 2007. Antibacterial activity and biomolecular composition of certain fresh water micro-algae from river Godavari (India). Science World Journal 2(3): 19-23.

Jiao, G., Yu, G., Zhang, J. & Ewart, H.S. 2011. Chemical structures and bio activities of sulfated polysaccharide from marine algae. Marine Drugs 9: 196-223.

Ji, S-G., Huo, K., Wang, J. & Pan, S-L. 2008. A molecular phylogenetic study of Huperziaceae based on chloroplast rbcL and psbA-trnH sequences. Journal of Systematics and Evolution 46(2): 213-219.

John, D.M., Whitton, B.A. & Brook, A.J. 2011. The Freshwater Algal Flora of the British IsIes. Cambridge: The United Kingdom at the University Press.

Kadlubowska, J.Z. 1984. Süßwasserflora von Mitteleuropa. Band 16: Chlorophyta VIII-Conjugatophyceae I (Zygnemales). Stuttgart: Gustav Fischer Verlag.

Kim, J.H. 2015. New records of the genus Spirogyra (Zygnemataceae, Conjugatophyceae) in Korea. Journal of Ecology and Environment 38(4): 611-618.

Lailerd, N., Pongchaidecha, A., Amornlerdpison, D. & Peerapornpisal, Y. 2009. Beneficial effects of Spirogyra neclecta extract on glycemic and lipidemic status in streptozotocin-induced diabetic rats fed a diet enriched in fat. Annals of Nutrition and Metabolism 55(S1): 709.

Jerez-Martel, I., García-Poza, S., Rodríguez-Martel, G., Rico, M., Afonso-Olivares, C. & Gómez-Pinchetti, J.L. 2021. Phenolic profile and antioxidant activity of crude extracts from microalgae and cyanobacteria strains. Advances in Food Science 2017: 2924508.

Nozaki, K. 2023. Morphological characteristics of Spirogyra neglecta (Zygnemataceae) collected from spring water in the urban area of Nagoya City, Aichi, Japan. Rikunomizu (Limnology in Tokai Region of Japan) 97: 35-43.

Peerapornpisal, Y., Amornlerdpison, D., Jamjai, U., Taesotikul, T., Pongpaibul, Y., Nualchareon, M. & Kanjanapothi, D. 2010. Antioxidant and anti-inflammatory activities of brown marine alga, Padina minor Yamada. Chiang Mai Journal of Science 37(3): 507-516. [in Thai]

Rattanabhorn, J., Amornlerdpison, D. & Chimsook, T. 2013. Phytochemical screening, antioxidant activity and total phenolic content of Spirogyra spp. Advanced Materials Research 699: 693-697.

Simons, J. 1987. Spirogyra species and accompanying algae from dune waters in the Netherlands. Acta Bot. Neerl. 36(1): 13-31.

Simons, J. & Van Beem, A.P. 1990. Spirogyra species and accompanying algae from pools and ditches in The Netherlands. Aquatic Botany 37(3): 247-269.

Stancheva, R., Hall, J.D., McCourt, R.M. & Sheath, R.G. 2013. Identity and phylogenetic placement of S pirogyra species (Zygnematophyceae, Charophyta) from California streams and elsewhere(1). Journal of Phycology 49(3): 588-607.

Takano, T., Ikegaya, H., Ikeda, K., Nozaki, H. & Sakayama, H. 2022. Morphology, taxonomy and phylogenetic positions of two Spirogyra species (Zygnematophyceae, Streptophyta) with replicate and semi-replicate transverse walls from Japan. Phycologia 61(4): 425-435.

Takano, T., Higuchi, S., Ikegaya, H., Matsuzaki, R., Kawachi, M., Takahashi, F. & Nozaki, H. 2019. Identifcation of 13 Spirogyra species (Zygnemataceae) by traits of sexual reproduction induced under laboratory culture conditions. Scientific Reports 9: 7458. DOI: 10.1038/s41598-019-43454-6

Timoshkin, O.A., Bondarenko, N.A., Volkova, Y.A., Tomberg, I.V., Vishnyakov, V.S. & Malnik, V.V. 2015. Mass development of green filamentous algae of the genera Spirogyra and Stigeoclonium (Chlorophyta) in the littoral zone of the Southern part of Lake Baikal. Hydrobiological Journal 51(1): 13-23.

Thiamdao, S. 2011. Diversity of edible freshwater macroalgae in Thailand during 2007-2008. PhD. Thesis in Biology, Chiang Mai University, Chiang Mai (Unpublished).

Thumvijit, T., Taya, S., Punvittayagul, C., Peerapornpisal, Y. & Wongpoomchai, R. 2014. Cancer chemopreventive effect of Spirogyra neglecta (Hassall) Kützing on diethylni trosamine-induced hepatocarcinogenesis in rats. Asian Pacific Journal of Cancer Prevention 15(4): 1611-1616.

Thumvijit, T., Thuschana, W., Amornlerdpison, D., Peerapornpisal, Y. & Wongpoomchai, R. 2013a. Evaluation of hepatic antioxidant capacities of Spirogyra neglecta (Hassall) Kützing in rats. Interdisciplinary Toxicology 6(3): 152-156.

Thumvijit, T., Inboot, W., Peerapornpisal, Y., Amornlerdpison, D. & Wongpoomchai, R. 2013b. The antimutagenic and antioxidant properties of Spirogyra neglecta (Hassall) Kützing. Journal of Medicinal Plants Research 7(34): 2494-2500.

Tipnee, S., Ramaraj, R. & Unparom, Y. 2015. Nutritional evaluation of edible freshwater green macroalga Spirogyra varians. Emergent Life Sciences Research 1(2): 1-7.

Townsend, S.A., Schult, J.H., Douglas, M.M. & Skinner, S. 2008. Does the Redfield ratio infer nutrient limitation in the macroalga Spirogyra fluviatilis. Freshwater Biology 53: 509-520.

Tuney, I., Cadirci, B.H., Unal, D. & Sukatar, A. 2006. Antimicrobial activities of the extracts of marine algae from the Coast of Urla (Izmir, Turkey). Turkish Journal of Biology 30(3): 10.

Turkmen, N., Sari, F. & Velioglu, Y.S. 2006. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin - Ciocalteu methods. Food Chemistry 99: 835-841.

Vlachos, V., Critchley, A.T. & Holy, A.V. 1997. Antimicrobial activity of extracts from selected southern African marine macroalgae. South African Journal of Science 93: 328-332.

Volkova, E.A., Bondarenko, N.A. & Timoshkin, O.A.Y. 2018. Morphotaxonomy, distribution and abundance of Spirogyra (Zygnematophyceae, charophyta) in lake baikal, East Siberia. Phycologia 57(3): 298-308.

Wongsawad, P. & Peerapornpisal, Y. 2014. Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers. Saudi Journal of Biological Sciences 21(5): 505-510.

Yosboonruang, A., Duangjai, A., Amormlerdpison, D. & Viyoach, J. 2020. Screening for biological activities of Spirogyra neglecta water extract. Walailak Journal of Science and Technology 17(4): 359-368.

 

*Pengarang untuk surat-menyurat; email: prasertsintippawan@gmail.com

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

   

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